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Jackson二抗|011-000-120*
將正常血清脂質(zhì)提取以提高透明度,對(duì)含有die氮化鈉的磷酸鹽緩沖鹽水(PBS)透析,并冷凍干燥。 強(qiáng)烈建議在PBS中稀釋至5%(v / v)的正常血清作為阻斷試劑,以減少非特異性,保守序列和/或Fc受體結(jié)合的背景。 使用來(lái)自與標(biāo)記抗體相同宿主的稀釋的正常血清獲得*結(jié)果,作為添加*抗體之前的單獨(dú)孵育步驟。
Jackson二抗|011-000-120*
Physical State: Freeze-dried solid
Storage:
Storage and Rehydration: Store freeze-dried solid at 2-8°C. Rehydrate with the indicated volume of dH2O and centrifuge if not clear. Prepare working dilution on day of use. Product is stable for about 6 weeks at 2-8°C as an undiluted liquid.
Extended Storage after Rehydration: Aliquot and freeze undiluted product at -20°C or below. Avoid repeated freezing and thawing.
Expiration date: one year from date of rehydration. The expiration date may be extended if test results are acceptable for the intended use.
Preservative: 0.05% Sodium Azide
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